Effect of Butylated Hydroxytoluene on Sperm Viability and Acrosomal Integrity in Cryopreserved Hariana Bull Semen
نویسندگان
چکیده
The present study was aimed to see the effect of butylated hydroxytoluene (BHT) as semen additives during cryopreservation of Hariana Bull semen. Twenty four ejaculates from two Hariana bulls extended with glycerolated egg yolk tris (GEYT). Extended samples were split into three aliquots. One aliquot was kept as control while other two aliquots were supplemented with 0.5 mM (T1) and 1.0 mM (T2) of BHT. All semen samples were equilibrated and cryopreserved in liquid nitrogen vapours and subsequently plunged in liquid nitrogen for preservation. The effect of BHT was determined by assessment of sperm viability and acrosomal integrity at three stages i.e. after dilution, pre-freeze and post –thaw. Our study revealed that addition of 1.0 mM of BHT results in a signifi cant (p<0.05) improvement in sperm viability and acrosomal integrity during cryopreservation.
منابع مشابه
Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls
AIM This study was aimed to see the beneficial effect of butylated hydroxytoluene (BHT) as a semen additive of Hariana bull semen. MATERIALS AND METHODS The study was carried out in Hariana bulls. Twenty-four ejaculates from two bulls were used for this study. Each ejaculate was extended with standard glycerolated egg yolk tris extender and supplemented with BHT at two concentrations as 0.5 m...
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